LOCUS       sequence-92888-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic 
            tracing and high-level gene expression in neurons.
  JOURNAL   Nat Protoc. 2010 Mar;5(3):595-606. doi: 10.1038/nprot.2009.248. Epub
            2010 Mar 4.
  PUBMED    20203674
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2009.248"; journalName: "Nat Protoc"; date: 
            "2010-03"; volume: "5"; issue: "3"; pages: "595-606"
COMMENT     Sequence Label: cSPBN-4GFP
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     80..96
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     80..96
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(119..166)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     162..180
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgct ctagaactag tggatccgga tatagttcct cctttcagca
      121 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
      181 tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatcc ccgccctccc
      241 ttagccatcc gagtggacgt gcgtcctcct tcggatgccc aggtcggacc gcgaggaggt
      301 ggagatgcca tgccgaccca cgcttaacaa ataaacaaca aaaatgagaa aaacaatcaa
      361 acaaccaaag gttcagattt aggatcttgt ttttttcaag atacatcaca caagagtctt
      421 agcatgggca ggcttccagg agtatccggt tcacaggcaa ctgtagtcta gtagggatga
      481 tctagatctg atatcctcta gggtgatcca cctgttgtac ctatgaaggt gtctttccac
      541 ttctctggat agatccttga tgctgccaac gagtctggta gtcttcacta tcttgtaaat
      601 caacctgatc cagtgagatg acagactcag gctagaatta caggctaccc ttttgaacac
      661 tgaggtgtcg ttggaccaac tccaagatag ataaacgttc cctcgaatga cttgctttct
      721 gaagtaataa gttataggtc tgttatacag gtcgtgaagt cttgcgaagc tagggacgtc
      781 acctaaagca gtagatagat acatcatagt actgcaacat atgttgaagt gcctcaggat
      841 tttgggatca gacggtggat agaacgaggg gtcagttagg ggtttggaaa cgttgaagac
      901 tctgttggag aaaactatca tgatggctat aatgatagcc actttagaca gagttcccct
      961 ctgtaactca agatcatcaa ccatcttgtg gactagaaaa gattctacat cactgtcaat
     1021 cagagttatg atcatctcat tg
//
