LOCUS       sequence-92907-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd* generation lentiviral packaging plasmid. (*See comments 
            section.) Can be used with 2nd and 3rd generation transfer vectors. 
            Use in conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pCMV-dR8.2 dvpr
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..153
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ccttggcact tatctgggac gatctgcgga gcctgtgcct
      121 cttcagctac caccgcttga gagacttact cttgattgta acgaggattg tggaacttct
      181 gggacgcagg gggtgggaag ccctcaaata ttggtggaat ctcctacaat attggagtca
      241 ggagctaaag aatagtgctg ttagcttgct caatgccaca gccatagcag tagctgaggg
      301 gacagatagg gttatagaag tagtacaagg agcttgtaga gctattcgcc acatacctag
      361 aagaataaga cagggcttgg aaaggatttt gctataagat gggtggcaag tggtcaaaaa
      421 gtagtgtgat tggatggcct actgtaaggg aaagaatgag acgagctgag ccagcagcag
      481 atggggtggg agcagtatct cgagacctag aaaaacatgg agcaatcaca agtagcaata
      541 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
      601 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
      661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
      721 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
      781 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
      841 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
      901 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
      961 gcctagcatt tcatcacatg gcccgagagc tgcatccgga gtacttcaag aactgctgag
     1021 cggccgcccc
//
