LOCUS       sequence-92917-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-neo
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..101
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aggatccgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat gacggtgatt
       61 ataaagatca tgacatcgat tacaaggatg acgatgacaa gctcatggac aagaagtact
      121 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      181 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
      241 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
      301 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
      361 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
      421 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
      481 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
      541 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
      601 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
      661 tccaactggt tcagacttac aatcagctt
//
