LOCUS       sequence-92927-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging 
            (the recipient vector can be TetON 3G promoter system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1658-dCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttcacctcg cggataagtt tgtcgttttc gtcgtacttg gtattcatgc gtgagtcaag
       61 gatttgggcg acatgtttag taatctgccg agtctccaca agttgccgtt taatgaatcc
      121 tgctttgtcc agttcgctca gtccgcccct ctcagcctta gtgagattgt cgaacttccg
      181 ctgagtgatc agctttgcgt tcagaagctg gcgccaatag ttcttcatct ttttcacgac
      241 ttcctctgag ggcacgttat ctgactttcc ccggttcttg tctgagcgag tcagcacctt
      301 attgtcaatt gagtcgtctt tgaggaagct ctgagggacg atggcgtcca cgtcgtagtc
      361 tgagagcctg ttgatatcaa gctcttggtc cacatacata tcgcgtccat tttgaaggta
      421 gtacagatag agcttctcat tctgaagctg ggtgttttcc accgggtgct ctttaaggat
      481 ctgtgacccg agctccttaa tcccttcctc aattcttttc atccgctccc tgctgttctt
      541 ctgtcccttc tgggtagtct ggttttccct ggccatctcg atgacaatgt tctctggttt
      601 atggcgaccc atcaccttga ccagctcgtc cacgactttc acggtctgga gaataccctt
      661 cttaatagcg ggtgaaccag ccagatttgc gatatgctcg tgcagtgagt caccttgtcc
      721 agacacttgg gccttctgga tgtcctcctt aaaggtcagg gaatcatcat ggataagttg
      781 catgaagttg cgattagcga atccgtcgct cttaaggaaa tcaagaatgg tctttccgct
      841 ctgtttgtcc cggattccgt tgatgagttt gcggctaagg cgtccccatc cggtatatct
      901 tctccgttta agttgcttca tcactttatc gtc
//
