LOCUS       sequence-92933-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector, integration at thrC, ampr, specr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS4S
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      171..242
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      258..285
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    292..312
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
       61 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      121 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac
      181 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      241 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagtag
      301 cggccgctgc aggataaaaa atttagaagc caatgaaatc tataaataaa ctaaattaag
      361 tttatttaat taacaactat ggatataaaa taggtactaa tcaaaatagt gaggaggata
      421 tatttgaata catacgaaca aattaataaa gtgaaaaaaa tacttcggaa acatttaaaa
      481 aataacctta ttggtactta catgtttgga tcaggagttg agagtggact aaaaccaaat
      541 agtgatcttg actttttagt cgtcgtatct gaaccattga cagatcaaag taaagaaata
      601 cttatacaaa aaattagacc tatttcaaaa aaaataggag ataaaagcaa cttacgatat
      661 attgaattaa caattattat tcagcaagaa atggtaccgt ggaatcatcc tcccaaacaa
      721 gaatttattt atggagaatg gttacaagag ctttatgaac aaggatacat tcctcagaag
      781 gaattaaatt cagatttaac cataatgctt taccaagcaa aacgaaaaaa taaaagaata
      841 tacggaaatt atgacttaga ggaattacta cctgatattc cattttctga tgtgagaaga
      901 gccattatgg attcgtc
//
