LOCUS       sequence-92937-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector (pT7) for untagged expression as well as fusions 
            to His6-SUMO or His6 tags. Designed for single or multiple fragment 
            cloning by yeast homologous recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Holmberg MA, Gowda NK, Andreasson C
  TITLE     A versatile bacterial expression vector designed for single-step 
            cloning of multiple DNA fragments using homologous recombination.
  JOURNAL   Protein Expr Purif. 2014 Jun;98:38-45. doi: 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12.
  PUBMED    24631626
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2014-06"; volume: "98:38-45. doi"; pages: " 
            10.1016/j.pep.2014.03.002. Epub 2014 Mar 12"
COMMENT     Sequence Label: pSUMO-YHRC
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..526
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 agggattttg ggggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
       61 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
      121 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
      181 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
      241 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
      301 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
      361 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
      421 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
      481 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatcgctt gcctcgtccc
      541 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      601 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      661 tataatcatt
//
