LOCUS       sequence-92966-e       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK101
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7612..8322
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(9204..9222)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9290..9394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9395..9463,9464..10255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9613..9632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10426..11014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10915..10934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(11102..11119,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11102..11119
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taactcagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggatccat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tatggtgtct
     7621 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     7681 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     7741 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     7801 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
     7861 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     7921 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     7981 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     8041 tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     8101 atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga
     8161 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg
     8221 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     8281 agatactgcg acctccctag caaactgggg cacaaactta atggaggcgg aggcggaggc
     8341 ggaggcatgt acgtgagatt tgaggttcct gaggacatgc agaacgaagc tctgagtctg
     8401 ctggagaagg ttagggagag cggtaaggta aagaaaggta ccaacgagac gacaaaggct
     8461 gtggagaggg gactggcaaa gctcgtttac atcgcagagg atgttgaccc gcctgagatc
     8521 gttgctcatc tgcccctcct ctgcgaggag aagaatgtgc cgtacattta cgttaaaagc
     8581 aagaacgacc ttggaagggc tgtgggcatt gaggtgccat gcgcttcggc agcgataatc
     8641 aacgagggag agctgagaaa ggagcttgga agccttgtgg agaagattaa aggccttcag
     8701 aagaagctta gccatggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc
     8761 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     8821 atcaatgtgt aactcgacgc gccccgcttg aagtctttaa ttaaaccgct tgaagtcttt
     8881 aattaaaccg cttgaagtct ttaattaaac cgcttgaagt ctttaattaa agctagttac
     8941 cctcgaggaa gcgcagtaca taatgctgcg cagtgttgcc acataaccac tatattaacc
     9001 atttatctag cggacgccaa aaactcaatg tatttctgag gaagcgtggt gcataatgcc
     9061 acgcagcgtc tgcataactt ttattatttc ttttattaat caacaaaatt ttgtttttaa
     9121 catttcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaggggaatt cgagctcggg
     9181 aattaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9241 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9361 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9421 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9481 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9541 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9601 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     9661 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9721 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9781 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9841 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9901 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9961 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10021 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10081 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10141 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10201 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10261 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10321 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10381 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10441 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10501 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10561 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10621 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10681 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10741 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10801 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10861 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10921 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10981 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcga gctcgtatgg
    11041 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
    11101 gatttaggtg acactatag
//
