LOCUS       sequence-92980-e        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1021
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..87
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             124..147
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(183..199)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(183..199)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttcgagctcg gtacccgggg atccctcgag gtcgacctgc agggggacca tggtctcagc
       61 gcttggtcgc atccacaatt tgagaaaggt ggaggttccg gaggtggatc gggaggtgga
      121 tcgtggagcc acccgcagtt cgaaaaataa taagcttgat atcgaattcc tgcagcccgg
      181 gggatccact agttctagag cggccgcgga acgatcattc actatatata tatcaattta
      241 tatatacgta tgtgtaattg aagaaagata cgttttttcc tctattgaga ggcctgctgg
      301 atgaatagct ttaccttttc taaatccttg ataccatcag tctctactcc tccacttaca
      361 tcgacaccga tcgcatttgg taacatatta atggcaacag aaacgttatc aggattcaat
      421 ccaccagcga taatgaattt tatctcggga tgacttgcag accaactgga aattgcactc
      481 caattcaatt tctcaccagt gccaccttca ccagaatcga acaacgtcag cacattgtct
      541 acgtgttcat acaggtccag tagtaattca caatcctgtg ggaactggaa cctcttaatg
      601 attggaattg aagatgggat caaagatctg tattctttaa tatcttcatc tccatgtaat
      661 tgtatcacat ctagattata ttcgtggtac agttgaagga catcatcaac ggactgattt
      721 ctaaacaccc cgactagttt agtacctttc acgttctctt gttggtgaac tgcagttgaa
      781 atacctttcg caacagatga gtcaatggtt cttttcctac cgggaacaca aatgatacct
      841 aagtaatcag caccatcatc cacagcagtc tttgcagctt caacggtttg caaaccacac
      901 actttaacga gcatcacagt agatttcgca agagggtagc tcgctcagag tacccaagta
      961 aatgattagt aaactgatgt ttgatagttc aatttttcaa tgaaataacc ttatattaaa
     1021 attgatatta ctattataca aaaataaaga ataaaggatt tgagtttata cataaaatac
     1081 cattattatt tgttcagtga gagataccgg ggtatatggg atgtgtgtag tgataccatg
     1141 caatcatgta tcaaacatgg gccctcgctc cgaccgtgcg gcatgcaagc t
//
