LOCUS       sequence-92982-e        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1022
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..88
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             125..148
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(184..200)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..200)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(239..436)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1255..1598)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 attcgagctc ggtacccggg gatccctcga ggtcgacctg cagggggacc atggtctcag
       61 cgcttggtcg catccacaat ttgagaaagg tggaggttcc ggaggtggat cgggaggtgg
      121 atcgtggagc cacccgcagt tcgaaaaata ataagcttga tatcgaattc ctgcagcccg
      181 ggggatccac tagttctaga gcggccgctg gatggcggcg ttagtatcga atcgacagca
      241 gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc gcacttaact
      301 tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta acatttgatt
      361 aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg aaaacaagaa
      421 tctttttatt gtcagtactg attataattg gccagtcttt ttcaaataag cattccaacc
      481 agcatctcta taccttttac cttcaatatc tggatctctt cctttaccaa acaatcctct
      541 accaacaatg ataatatcag ttccagtgct aacaacttca tcaacagttc tatattgttg
      601 tcctaatcca tcacctttat catctaatcc aactccaggt gtcataataa gccaatcaaa
      661 tccttcttct tgtccaccca tatcacgttg ggcaataaat ccaataacaa attccttatc
      721 ggatttagca atttcaacag ttttttgaga atattctcca tatgctaatg atcccactga
      781 tgataattca gctaacatca ataaccctct tggctcttgg ttggtggtgg tttctttagc
      841 tccctgtttt aatccttcaa ctactccatt cccagtgaca ccatgagcat tagtaatatc
      901 tgcccaacta ctaattttat aaactccacc aatatattgt ttcttcacgg tattaccaat
      961 atcagcaaat tttctatctt caaaaatcat aaattgatgt ttacgtgaaa gttctaataa
     1021 tggttcaata gtggattcat aggaaaaatc attgattata tcaatatgag tcttgattaa
     1081 gcatacataa ggacccaatt tatcaattaa ttcaaggaat tccttagtgg tatcaacatc
     1141 aattgatgca cataaattgg ttttcttcag ttccattaat cgaaataatc gttgtgctac
     1201 tggtgaggca tgagtttctg ctctctcact ataggtctta gtgttgactg tcatggttgt
     1261 ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg aagtatatga
     1321 aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg cgcggcgtgg
     1381 ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtccg cagcgaggag
     1441 ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa tgattataca
     1501 tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga gctgcgcacg
     1561 tcaagactgt caaggagggt attctgggcc tccatgtcgc tggccgggtg acccggcggg
     1621 gacgaggcaa gctgtcgatc aggcgcatgc aagct
//
