LOCUS       sequence-92994-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-I2
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..100
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggatccgttt agtgaaccgt cagaattaac catggactac aaagaccatg acggtgatta
       61 taaagatcat gacatcgatt acaaggatga cgatgacaag ctcatggaca agaagtactc
      121 cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa
      181 ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa
      241 cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag
      301 aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt
      361 tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt
      421 ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt
      481 ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac
      541 tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg
      601 acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat
      661 ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt
      721 tgacgccaaa gcaatcctga
//
