LOCUS       sequence-93-expo        6434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_11
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(256..278)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3874..3896)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3996..4015
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4093..4111)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4179..4283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4284..4352,4353..5144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4502..4521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5315..5903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5804..5823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6057..6074
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6191..6212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6227..6232,6233..6250,6251..6257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6265..6281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6270..6292
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6289..6305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6289..6305
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tgggtgaaga
      181 ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg cgctgtatgg
      241 cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      301 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      361 cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact aactaacttg
      421 ttctgacata attttcagct tgaatggcga atggcgcttt gcctggtttc cggcaccaga
      481 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
      541 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
      601 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
      661 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
      721 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg taagtttaat
      781 taagttgata ctaactaaca agatctgatt aattttcagg acagtcgttt gccgtctgaa
      841 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
      901 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
      961 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
     1021 tttaatgatg atttctcccg cgctgtactg gaggctgaag tccaggtaag tttaaacagg
     1081 atcttactaa ctaacatgct aacactgaat tttcagatgt gcggcgagtt gcgtgactac
     1141 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
     1201 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
     1261 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
     1321 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     1381 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     1441 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     1501 caggatgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagatc
     1561 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     1621 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     1681 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     1741 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     1801 tcgctgggga atggtaagtt taaacagttg aatactaact aacggagatc tttgaaattt
     1861 tcagaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
     1921 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
     1981 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
     2041 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgattctttg cgaggtaagt
     2101 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagtacgc tcacgcgatg
     2161 ggcaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta
     2221 cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc
     2281 aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt
     2341 atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgtaagttta aacaataacc
     2401 tactaactaa cgtagataat ttaaattttc aggctgacgg aagcaaaaca ccagcagcag
     2461 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     2521 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     2581 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     2641 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     2701 accgcatggt cagaagccgg gcacatcagc gcatggcagc agtggaggta agtttaaaca
     2761 agatcctact aactaactct acattgatga attttcagac tggcggaaaa cctcagtgtg
     2821 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
     2881 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
     2941 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
     3001 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcggt
     3061 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcaga acgctggaag
     3121 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct
     3181 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc
     3241 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
     3301 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta
     3361 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
     3421 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtagtaagt ttaaacttga
     3481 tagtactaac taacatgttt catttaaatt ttcagtaccc cgtacgtctt cccgagcgaa
     3541 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
     3601 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat
     3661 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
     3721 ggcgacgact cctggagccc gtcagtatcg gcggaattcc aactgagcgc cggtcgctac
     3781 cattaccaac ttgtctggtg tcaaaaataa taggcctact agtcggccgt acgggccctt
     3841 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3901 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3961 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     4021 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     4081 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4141 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4501 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5701 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5941 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6001 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6061 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6301 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
     6361 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa
     6421 cttggaaatg aaat
//
