LOCUS       sequence-93001-e        1671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct for single molecule study.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Springer
  TITLE     Springer lab ReaLiSM plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ReaLiSM A1-43-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..1671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             640..660
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1648..1665
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagtgctccg gagacatctc ggaaccgccg ttgcacgatt tctactgcag caggctactg
       61 gacctggtct tcctgctgga tggctcctcc aggctgtccg aggctgagtt tgaagtgctg
      121 aaggcctttg tggtggacat gatggagcgg ctgcgcatct cccagaagtg ggtccgcgtg
      181 gccgtggtgg agtaccacga cggctcccac gcctacatcg ggctcaagga ccggaagcga
      241 ccatcagagc tgcggcgcat tgccagccag gtgaagtatg cgggcagcca ggtggcctcc
      301 accagcgagg tcttgaaata cacactgttc caaatcttca gcaagatcga ccgccctgaa
      361 gcctcccgca tcgccctgct cctgatggcc agccaggagc cccaacggat gtcccggaac
      421 tttgtccgct acgtccaggg cctgaagaag aagaaggtca ttgtgatccc ggtgggcatt
      481 gggccccatg ccaacctcaa gcagatccgc ctcatcgaga agcaggcccc tgagaacaag
      541 gccttcgtgc tgagcagtgt ggatgagctg gagcagcaaa gggacgagat cgttagctac
      601 ctctgtgacc ttgcccctga agcccctcct ggtaccgggg agaatcttta ttttcaaggc
      661 caccatcacc atcaccatgg atccagctcc agcggcagct ccagctccgg atccagctcc
      721 agcgggacca ctggttggag aggtggccat gttggtaccc accccatctg tgaggtctcc
      781 aaagtggcca gccacctaga agtgaactgt gacaagagga atctgacagc gctgcctcca
      841 gacctgccga aagacacaac catcctccac ctgagtgaga acctcctgta caccttctcc
      901 ctggcaaccc tgatgcctta cactcgcctc actcagctga acctagatag gtgcgagctc
      961 accaagctcc aggtcgatgg gacgctgcca gtgctgggga ccctggatct atcccacaat
     1021 cagctgcaaa gcctgccctt gctagggcag acactgcctg ctctcaccgt cctggacgtc
     1081 tccttcaacc ggctgacctc gctgcctctt ggtgccctgc gtggtcttgg cgaactccaa
     1141 gagctctacc tgaaaggcaa tgagctgaag accctgcccc cagggctcct gacgcccaca
     1201 cccaagctgg agaagctcag tctggctaac aacaacttga ctgagctccc cgctgggctc
     1261 ctgaatgggc tggagaatct cgacaccctt ctcctccaag agaactcgct gtatacaata
     1321 ccaaagggct tttttgggtc ccacctcctg ccttttgctt ttctccacgg gaacccctgg
     1381 ttatgcaact gtgagatcct ctattttcgt cgctggctgc aggacaatgc tgaaaatgtc
     1441 tacgtatgga agcaaggtgt ggacgtcaag gccatgacct ctaacgtggc cagtgtgcag
     1501 tgtgacaatt cagacaagtt tcccgtctac aaatacccag gaaaggggtg ccccaccctt
     1561 ggtgatgaag gtgacacaga cctatatgat tactacccag aagaggacac tgagggcgat
     1621 aaggtgcgtg gtaccaaggg ctgcggtcat caccatcacc atcacgcgta a
//
