LOCUS       sequence-93012-e        1620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Kim J, Zhang CZ, Zhang X, Springer TA
  TITLE     A mechanically stabilized receptor-ligand flex-bond important in the
            vasculature.
  JOURNAL   Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295.
  PUBMED    20725043
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-19- 19"; volume: "466"; issue: "7309"; 
            pages: "992-5"
COMMENT     Sequence Label: ReaLiSM A1-26link-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..1620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             640..660
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1597..1614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagtgctccg gagacatctc ggaaccgccg ttgcacgatt tctactgcag caggctactg
       61 gacctggtct tcctgctgga tggctcctcc aggctgtccg aggctgagtt tgaagtgctg
      121 aaggcctttg tggtggacat gatggagcgg ctgcgcatct cccagaagtg ggtccgcgtg
      181 gccgtggtgg agtaccacga cggctcccac gcctacatcg ggctcaagga ccggaagcga
      241 ccatcagagc tgcggcgcat tgccagccag gtgaagtatg cgggcagcca ggtggcctcc
      301 accagcgagg tcttgaaata cacactgttc caaatcttca gcaagatcga ccgccctgaa
      361 gcctcccgca tcgccctgct cctgatggcc agccaggagc cccaacggat gtcccggaac
      421 tttgtccgct acgtccaggg cctgaagaag aagaaggtca ttgtgatccc ggtgggcatt
      481 gggccccatg ccaacctcaa gcagatccgc ctcatcgaga agcaggcccc tgagaacaag
      541 gccttcgtgc tgagcagtgt ggatgagctg gagcagcaaa gggacgagat cgttagctac
      601 ctctgtgacc ttgcccctga agcccctcct ggtaccgggg agaatcttta ttttcaaggc
      661 ggatccagct ccagcaccac tggttggaga ggtggccatg ttggtaccca ccccatctgt
      721 gaggtctcca aagtggccag ccacctagaa gtgaactgtg acaagaggaa tctgacagcg
      781 ctgcctccag acctgccgaa agacacaacc atcctccacc tgagtgagaa cctcctgtac
      841 accttctccc tggcaaccct gatgccttac actcgcctca ctcagctgaa cctagatagg
      901 tgcgagctca ccaagctcca ggtcgatggg acgctgccag tgctggggac cctggatcta
      961 tcccacaatc agctgcaaag cctgcccttg ctagggcaga cactgcctgc tctcaccgtc
     1021 ctggacgtct ccttcaaccg gctgacctcg ctgcctcttg gtgccctgcg tggtcttggc
     1081 gaactccaag agctctacct gaaaggcaat gagctgaaga ccctgccccc agggctcctg
     1141 acgcccacac ccaagctgga gaagctcagt ctggctaaca acaacttgac tgagctcccc
     1201 gctgggctcc tgaatgggct ggagaatctc gacacccttc tcctccaaga gaactcgctg
     1261 tatacaatac caaagggctt ttttgggtcc cacctcctgc cttttgcttt tctccacggg
     1321 aacccctggt tatgcaactg tgagatcctc tattttcgtc gctggctgca ggacaatgct
     1381 gaaaatgtct acgtatggaa gcaaggtgtg gacgtcaagg ccatgacctc taacgtggcc
     1441 agtgtgcagt gtgacaattc agacaagttt cccgtctaca aatacccagg aaaggggtgc
     1501 cccacccttg gtgatgaagg tgacacagac ctatatgatt actacccaga agaggacact
     1561 gagggcgata aggtgcgtgg taccaagggc tgcggtcatc accatcacca tcacgcgtaa
//
