LOCUS       sequence-93014-e        1617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express VWF A1-GPIba ReaLiSM construct in mammalian cells  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Kim J, Zhang CZ, Zhang X, Springer TA
  TITLE     A mechanically stabilized receptor-ligand flex-bond important in the
            vasculature.
  JOURNAL   Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295.
  PUBMED    20725043
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-19- 19"; volume: "466"; issue: "7309"; 
            pages: "992-5"
COMMENT     Sequence Label: ReaLiSM A1-Hinge-GPIba+2cys
FEATURES             Location/Qualifiers
     source          1..1617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1597..1614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctgaaaccg gttgtatctc ggaaccgccg ttgcacgatt tctactgcag caggctactg
       61 gacctggtct tcctgctgga tggctcctcc aggctgtccg aggctgagtt tgaagtgctg
      121 aaggcctttg tggtggacat gatggagcgg ctgcgcatct cccagaagtg ggtccgcgtg
      181 gccgtggtgg agtaccacga cggctcccac gcctacatcg ggctcaagga ccggaagcga
      241 ccatcagagc tgcggcgcat tgccagccag gtgaagtatg cgggcagcca ggtggcctcc
      301 accagcgagg tcttgaaata cacactgttc caaatcttca gcaagatcga ccgccctgaa
      361 gcctcccgca tcgccctgct cctgatggcc agccaggagc cccaacggat gtcccggaac
      421 tttgtccgct acgtccaggg cctgaagaag aagaaggtca ttgtgatccc ggtgggcatt
      481 gggccccatg ccaacctcaa gcagatccgc ctcatcgaga agcaggcccc tgagaacaag
      541 gccttcgtgc tgagcagtgt ggatgagctg gagcagcaaa gggacgagat cgttagctac
      601 ctctgtgacc ttgcccctga agcccctcct cctactggta ccgggcccac aatcaagccg
      661 cctaagcctc caaaaccagc acctaacctc ctgggcggac cgggcaccgg tcaccccatc
      721 tgtgaggtct ccaaagtggc cagccaccta gaagtgaact gtgacaagag gaatctgaca
      781 gcgctgcctc cagacctgcc gaaagacaca accatcctcc acctgagtga gaacctcctg
      841 tacaccttct ccctggcaac cctgatgcct tacactcgcc tcactcagct gaacctagat
      901 aggtgcgagc tcaccaagct ccaggtcgat gggacgctgc cagtgctggg gaccctggat
      961 ctatcccaca atcagctgca aagcctgccc ttgctagggc agacactgcc tgctctcacc
     1021 gtcctggacg tctccttcaa ccggctgacc tcgctgcctc ttggtgccct gcgtggtctt
     1081 ggcgaactcc aagagctcta cctgaaaggc aatgagctga agaccctgcc cccagggctc
     1141 ctgacgccca cacccaagct ggagaagctc agtctggcta acaacaactt gactgagctc
     1201 cccgctgggc tcctgaatgg gctggagaat ctcgacaccc ttctcctcca agagaactcg
     1261 ctgtatacaa taccaaaggg cttttttggg tcccacctcc tgccttttgc ttttctccac
     1321 gggaacccct ggttatgcaa ctgtgagatc ctctattttc gtcgctggct gcaggacaat
     1381 gctgaaaatg tctacgtatg gaagcaaggt gtggacgtca aggccatgac ctctaacgtg
     1441 gccagtgtgc agtgtgacaa ttcagacaag tttcccgtct acaaataccc aggaaagggg
     1501 tgccccaccc ttggtgatga aggtgacaca gacctatatg attactaccc agaagaggac
     1561 actgagggcg ataaggtgcg taccgggggc tgcggtcatc accatcacca tcactaa
//
