LOCUS       sequence-93017-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His3 GST tagged WT p110 beta .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pBacPAK p110b WT
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..720
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     678..700
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             727..750
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cgtacgtttt gtataaaaaa acctataaat accatgggtc atcatcatca ccatcacggg
       61 tcgaccatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
      121 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      181 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      241 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      301 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      361 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      421 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      481 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      541 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      601 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      661 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      721 tcggatctgg aagttctgtt ccaggggccc ctaagcttgg atccctgcag gcctcgagtt
      781 cctcctgcta tggcagacaa ccttgacatc tgggcagtgg actcacagat tgcatccgat
      841 ggcgccatat ccgtcgattt ccttctgccc accgggattt atatccagtt ggaagtacct
      901 cgggaagcta ccatttctta tattaaacag atgttatgga agcaagttca caactacccg
      961 atgtttaacc tcctcatgga cat
//
