LOCUS       sequence-93019-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 from the human 
            PGK-1 gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
  TITLE     Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease 
            complexes induces targeted mutagenesis in a diverse array of human 
            cells.
  JOURNAL   Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
  PUBMED    24870050
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-05-29"; volume: "4:5105. doi"; pages: " 
            10.1038/srep05105"
COMMENT     Sequence Label: pAdSh.PGK.Cas9
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..77)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcggccgct cgagctctgt acatgtccgc ggtcgggtag ggatcgaacc ctttcacacc
       61 ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt
      121 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
      181 gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
      241 aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc
      301 ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt
      361 ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg
      421 aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata gtggctggcc
      481 agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg
      541 cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga
      601 agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg
      661 gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt
      721 ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact
      781 gtaggagaat cgaatccgcc gtatttcttg gggtcccaat cttttttgcg tgcgatcagc
      841 ttgtcgctgt tccttttcgg gaggatactt tccttgga
//
