LOCUS       sequence-93027-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene 
            transfer, hypercholesterolemia and atherosclerosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Bjorklund MM, Hollensen AK, Hagensen MK, Dagnaes-Hansen F, 
            Christoffersen C, Mikkelsen JG, Bentzon JF
  TITLE     Induction of atherosclerosis in mice and hamsters without germline 
            genetic engineering.
  JOURNAL   Circ Res. 2014 May 23;114(11):1684-9. doi: 
            10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27.
  PUBMED    24677271
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.114.302937"; journalName: "Circ Res"; date: 
            "2014-05-23- 23"; volume: "114"; issue: "11"; pages: "1684-9"
COMMENT     Sequence Label: pAAV/D374Y-hPCSK9
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             421..432
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 acagatggct ggcaactaga aggcacaggc cggcctcact ggagctcctg ggaggcctgc
       61 gccaggtgcc ggctccggca gcagatggca acggctgtca cggcctcttc gctggtgctg
      121 cctgtagtgc tgacgtcccg gctcctgact acacacgtgt tgtctacggc gtaggccccc
      181 aggacgtggg aggtcccagg gagggcactg cagccagtca gggtccagcc ctcctcgcag
      241 gccacggtca cctgctcctg aggggccggg attccatgct ccttgacttt gcattccaga
      301 cctggggcat ggcagcagga agcgtggatg ctggcctccc tgtggcccac gcactggttg
      361 ggctgacctc gtggcctcag cacaggcggc ttgtgggtgc caaggtcctc cacctcccag
      421 tgggagctgc agcctgtgag gacgtggccc tgttggtggc agtggacacg ggtccccatg
      481 ctggcctcag ctggtggagc tgtgtggacg ctgcagttgg cctggggtag caggcagcac
      541 ctggcaatgg cgtagacacc ctcaccccca aaagcgttgt gggcccggca gaccagcttg
      601 cccccttggg cctccatgcg ctcgccccgc cgcttcccac tcctggagaa actggagcag
      661 ctcagcagct cctcatctgg ggcgcagcgg gcgatggctg tggccatccg tgtaggcccc
      721 gagtgtgctg accacacagt cctgcaaaac agctgccaac ctgccccatg ggtgctgggg
      781 ggcagggcgg ccaccaggtt gggggtcagt acccgctggt cctcagggaa ccaggcctca
      841 ttgatgacat ctttggca
//
