LOCUS       sequence-93038-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4NXJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MAC045-C12
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             453..470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(523..541)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      537..584
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(708..727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaaggagat ataccatgca tcatcatcat catcacagca gcggcagaga aaacttgtat
       61 ttccagggct atgacgagat tgaagaatta aagagcaaga atgaagtact aacaaattta
      121 ttaaataagc taatagcttt tgataagaaa agaatatttt tatatcctgt gaatgtacag
      181 ttagtacctg attatttgaa tgtcataaaa gaacctatgg attttacaac aatgaaacag
      241 aaacttcaga attttaaata taaaagtttt caagaatttg agaaagatgt tcttttaatt
      301 attaataatt gttatacata taatgaccca agtacaatat attataaatt tgctgaagat
      361 atagaaactt attataaaaa attaaatatt aaaattcaaa cgaaatatat gaacatacac
      421 ctatgatgac gaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      481 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      541 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      601 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      661 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      721 cttcccttcc ttctcgccca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      781 ccctttaggg ttccgattta
//
