LOCUS       sequence-93057-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: mEmerald-H2B-6
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(32..409)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(498..701)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(527..547)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat ccttagcgct ggtgtacttg gtgatggcct
       61 tagtaccctc ggacacggcg tgcttggcca actccccagg cagcagcagg cgcacggccg
      121 tctggatctc cctggaggtg atggtcgagc gcttgttgta atgcgccagg cgggaagcct
      181 cacctgcgat gcgctcgaaa atgtcgttca caaacgaatt catgatgccc atggccttgg
      241 acgaaatgcc ggtgtcaggg tggacctgct tcagaacctt gtacacatag atggaatagc
      301 tctccttgcg gctgcgcttg cgcttcttgc cgcctttctt ctgcgcctta gtcaccgcct
      361 tcttggagcc ctttttcggg gcgggagcag acttcgctgg ctctggcatg gtggcggtac
      421 cgtcgactgc agaattcgaa gcttgagctc gagatctgag tccggtagcg ctagcggatc
      481 tgacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc
      541 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      601 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      661 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      721 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg g
//
