LOCUS       sequence-93060-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant lacking the C-terminal and NLS domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1L-dC
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(661..684)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(752..771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(753..771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(816..867)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(845..865)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aatcttttat tttatcggcc ctctagatgc atgctcgagt tactttttca aagcctgaat
       61 tagattttgt ctatactctg ggtctatgca ccacaacgac cctttcccaa tactctgact
      121 cctctctttg tccactttct taaaacactt attcaatgat aaattgtgtc tcactgagtt
      181 tttccaccca gtaggtgcat ttgcaaaata cggaaaatgt tccaagatcc agttgtagat
      241 atccttcact ggcaggcgct tggttggaga gtcctcgatg gccataaata tgaggcagct
      301 gaaggagtag gggggtttgc agttggggtt ctgcctggca tcgtagggca tgtcagagtg
      361 ggcaggggat gggggggtgt catcgtccag gtcctggacg gggctgacac tcctgaggac
      421 cgactcccca aagctcttca gcaagttctt gctctcgtgc agccagttca ggttggtcag
      481 ctcttcatct tccatggccc cctcttctaa tcggatgtca ggcagagaaa agtcgaggtc
      541 atcgtcttcc tgaagggcct tggagaaacc gctgcccccg taacactgac tcagtccact
      601 ggagacacta attcctgagc tttctggctt cttactggga ggcatgactg gacccatgcc
      661 cttgtcgtca tcgtctttgt agtccatggc gaattccagc acactggcgg ccgttactag
      721 tggatccgag ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata
      781 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
      841 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttg
//
