LOCUS       sequence-93062-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-CHES1L mutant with ponctual mutation on histidine 164.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1H164A
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcgcca
      121 tggactacaa agacgatgac gacaagggca tgggtccagt catgcctccc agtaagaagc
      181 cagaaagctc aggaattagt gtctccagtg gactgagtca gtgttacggg ggcagcggtt
      241 tctccaaggc ccttcaggaa gacgatgacc tcgacttttc tctgcctgac atccgattag
      301 aagagggggc catggaagat gaagagctga ccaacctgaa ctggctgcac gagagcaaga
      361 acttgctgaa gagctttggg gagtcggtcc tcaggagtgt cagccccgtc caggacctgg
      421 acgatgacac ccccccatcc cctgcccact ctgacatgcc ctacgatgcc aggcagaacc
      481 ccaactgcaa acccccctac tccttcagct gcctcatatt tatggccatc gaggactctc
      541 caaccaagcg cctgccagtg aaggatatct acaactggat cttggaacat tttccgtatt
      601 ttgcaaatgc acctactggg tggaaaaact cagtgagagc caatttatca ttgaataagt
      661 gttttaagaa agtggacaaa gagaggagtc agagtattgg gaaagggtcg ttgtggtgca
      721 tagacccaga gtatagacaa aatctaattc aggctttgaa aaagacacct tatcacccac
      781 acccacacgt gttcaataca cctcccacct gtcctcaggc atatcaaagc acatcaggtc
      841 cacccatctg gccgggcagt accttcttca agagaa
//
