LOCUS       sequence-93089-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            tagRFP via P2A cleavage site. SFFV Promoter driven .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.SFFV.tRFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..756)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acaaattttg taatccagag gttgattgtc gacttaacgc gttcaattaa gtttgtgccc
       61 cagtttgcta gggaggtcgc agtatctggc cacagccacc tcgtgctgct cgacgtaggt
      121 ctctttgtcg gcctccttga ttctttccag tctgtggtcc acatagtaga cgccgggcat
      181 cttgaggttc ttagcgggtt tcttggatct gtatgtggtc ttgaagttgc agatcaggtg
      241 gcccccgccc acgagcttca gggccatgtc gcttctgcct tccaggccgc cgtcagcggg
      301 gtacagcatc tcggtgttgg cctcccagcc gagtgttttc ttctgcatca cagggccgtt
      361 ggatgggaag ttcacccctc tgatcttgac gttgtagatg aggcagccgt cctggaggct
      421 ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat gtggtgactc tctcccatgt
      481 gaagccctca gggaaggact gcttaaagaa gtcggggatg ccctgggtgt ggttgatgaa
      541 ggttctgctg ccgtacatga agctggtagc caggatgtcg aaggcgaagg ggagagggcc
      601 gccctcgacc accttgattc tcatggtctg ggtgccctcg tagggcttgc cttcgccctc
      661 ggatgtgcac ttgaagtggt ggttgttcac ggtgccctcc atatacagct tcatgtgcat
      721 gttctcctta atcagctctt cgcccttaga caccatgggg ccggggttct cctccacgtc
      781 gccggcctgc ttca
//
