LOCUS       sequence-93100-e        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of a fluorescent protein fragment derived from 
            Venus for BiFC and multicolor BiFC assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Shyu YJ, Liu H, Deng X, Hu CD
  TITLE     Identification of new fluorescent protein fragments for bimolecular 
            fluorescence complementation analysis under physiological 
            conditions.
  JOURNAL   Biotechniques. 2006 Jan . 40(1):61-6.
  PUBMED    16454041
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2006 Jan . 40(1):61-6."
COMMENT     Sequence Label: pBiFC-VC155
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          154..250
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             314..340
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             443..694
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     631..652
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    709..843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(733..752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     787..806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(954..970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(954..970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(967..989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    978..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1002..1008,1009..1026,1027..1032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
      121 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
      181 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
      241 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
      301 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
      361 aattcggtcg accgagatct ctcgaggtac ccgtccggcg tgcaaaatcc cgaacgacct
      421 gaaacagaaa gtcatgaacc acgacaagca gaagaacggc atcaaggcca acttcaagat
      481 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      541 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact
      601 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      661 cgggatcact ctcggcatgg acgagctgta caagtaagcg gccgcgggga tccagacatg
      721 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      841 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      901 ttttcggatc ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag
      961 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1021 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1081 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggcc
//
