LOCUS       sequence-93101-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses His-tagged B18R protein from Western vaccinia virus that 
            binds to and neutralizes type I IFNs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: pTNT-B18R-6His
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        129..147
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        153..171
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     153..170
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga tcttaaggct
      121 agagtactta atacgactca ctataggcta gcatttaggt gacactatag aatacaagct
      181 acttgttctt tttgcactcg agaattgcca ccatgggcac gatgaaaatg atggtacata
      241 tatatttcgt atcattattg ttattgctat tccacagtta cgccatagac atcgaaaatg
      301 aaatcacaga attcttcaat aaaatgagag atactctacc agctaaagac tctaaatggt
      361 tgaatccagc atgtatgttc ggaggcacaa tgaatgatat agccgctcta ggagagccat
      421 tcagcgcaaa gtgtcctcct attgaagaca gtcttttatc gcacagatat aaagactatg
      481 tggttaaatg ggaaaggcta gaaaaaaata gacggcgaca ggtttctaat aaacgtgtta
      541 aacatggtga tttatggata gccaactata catctaaatt cagtaaccgt aggtatttgt
      601 gcaccgtaac tacaaagaat ggtgactgtg ttcagggtat agttagatct catattagaa
      661 aacctccttc atgcattcca aaaacatatg aactaggtac tcatgataag tatggcatag
      721 acttatactg tggaattctt tacgcaaaac attataataa tataacttgg tataaagata
      781 ataaggaaat taatatcgac gacattaagt attcacaaac gggaaaggaa ttaattattc
      841 ataatccaga gttagaagat agcggaaga
//
