LOCUS       sequence-93105-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O61 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC02A-C10
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             792..809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttccctctag aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggcc agcagctgca gaaggaggag
      121 gaggcgcgca aggtgaagag cggcatccgc cagatgcgcc tcttcagcca ggacgagtgc
      181 gccaagatcg aggcccgcat tgacgaggtg gtgtcccgcg ctgagaaggg cctgtacaac
      241 gagcacacgg tggaccgggc cccactgcgc aacaagtact tcttcggcga aggctacact
      301 tacggcgccc agctgcagaa gcgcgggccc ggccaggagc gcctctaccc gccgggcgac
      361 gtggacgaga tccccgagtg ggtgcaccag ctggtgatcc aaaagctggt ggagcaccgc
      421 gtcatccccg agggcttcgt caacagcgcc gtcatcaacg actaccagcc cggcggctgc
      481 atcgtgtctc acgtggaccc catccacatc ttcgagcgcc ccatcgtgtc cgtgtccttc
      541 tttagcgact ctgcgctgtg cttcggctgc aagttccagt tcaagcctat tcgggtgtcg
      601 gaaccagtgc tttccctgcc ggtgcgcagg ggaagcgtga ctgtgctcag tggatatgct
      661 gctgatgaaa tcactcactg catacggcct caggacatca aggagcgccg agcagtcatc
      721 atcctcagga agacaagatt agatgcaccc cggttggaaa catgatgacg aagcttgcgg
      781 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gccccgaaaa
      841 ggaaagctga atttggctgc tgcccaccgc tgaaacaata actagcataa tccccttggg
      901 ggccttctta aa
//
