LOCUS       sequence-93106-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O61 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC02A-C10
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(735..755)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(768..785)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    838..854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttcgtcatc atgtttccaa ccggggtgca tctaatcttg tcttcctgag gatgatgact
      121 gctcggcgct ccttgatgtc ctgaggccgt atgcagtgag tgatttcatc agcagcatat
      181 ccactgagca cagtcacgct tcccctgcgc accggcaggg aaagcactgg ttccgacacc
      241 cgaataggct tgaactggaa cttgcagccg aagcacagcg cagagtcgct aaagaaggac
      301 acggacacga tggggcgctc gaagatgtgg atggggtcca cgtgagacac gatgcagccg
      361 ccgggctggt agtcgttgat gacggcgctg ttgacgaagc cctcggggat gacgcggtgc
      421 tccaccagct tttggatcac cagctggtgc acccactcgg ggatctcgtc cacgtcgccc
      481 ggcgggtaga ggcgctcctg gccgggcccg cgcttctgca gctgggcgcc gtaagtgtag
      541 ccttcgccga agaagtactt gttgcgcagt ggggcccggt ccaccgtgtg ctcgttgtac
      601 aggcccttct cagcgcggga caccacctcg tcaatgcggg cctcgatctt ggcgcactcg
      661 tcctggctga agaggcgcat ctggcggatg ccgctcttca ccttgcgcgc ctcctcctcc
      721 ttctgcagct gctggccctg gaaatacaag ttttctctgc cgctgctgtg atgatgatga
      781 tgatgcatgg tatatctcct tcttaaaagt taaacaaaat tatttctaga gggggaattg
      841 ttatccgctc acaa
//
