LOCUS       sequence-93108-e       10491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector that expresses catalytically inactive form of 
            Flag-tagged human USP7 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
  TITLE     Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation 
            of the INK4a tumour suppressor.
  JOURNAL   EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
            2010 Jul 2.
  PUBMED    20601937
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.129"; journalName: "EMBO J"; date: "2010-08-4- 
            4"; volume: "29"; issue: "15"; pages: "2553-65"
COMMENT     Sequence Label: pQFlag-USP7 CS puroR
FEATURES             Location/Qualifiers
     source          1..10491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2265..2288
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(5619..6192,6193..6195,6196..6204)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5786..5803)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6013..6032
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6224..6823
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6224..6243)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6720..6740
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7197..7622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7725..7747)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7883..7903)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7888..8217
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8068..8203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8239..8258
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8339..8356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8510..9098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8590..8609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9269..10060,10061..10129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9892..9911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10130..10234)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10302..10320
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10420..10491,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attggattac aaggatgacg
     2281 atgacaagac cggtatgaac caccagcagc agcagcagca gcagaaagcg ggcgagcagc
     2341 agttgagcga gcccgaggac atggagatgg aagcgggaga tacagatgac ccaccaagaa
     2401 ttactcagaa ccctgtgatc aatgggaatg tggccctgag tgatggacac aacaccgcgg
     2461 aggaggacat ggaggatgac accagttggc gctccgaggc aacctttcag ttcactgtgg
     2521 agcgcttcag cagactgagt gagtcggtcc ttagccctcc gtgttttgtg cgaaatctgc
     2581 catggaagat tatggtgatg ccacgctttt atccagacag accacaccaa aaaagcgtag
     2641 gattctttct ccagtgcaat gctgaatctg attccacgtc atggtcttgc catgcacaag
     2701 cagtgctgaa gataataaat tacagagatg atgaaaagtc gttcagtcgt cgtattagtc
     2761 atttgttctt ccataaagaa aatgattggg gattttccaa ttttatggcc tggagtgaag
     2821 tgaccgatcc tgagaaagga tttatagatg atgacaaagt tacctttgaa gtctttgtac
     2881 aggcggatgc tccccatgga gttgcgtggg attcaaagaa gcacacaggc tacgtcggct
     2941 taaagaatca gggagcgact agttacatga acagcctgct acagacgtta tttttcacga
     3001 atcagctacg aaaggctgtg tacatgatgc caaccgaggg ggatgattcg tctaaaagcg
     3061 tccctttagc attacaaaga gtgttctatg aattacagca tagtgataaa cctgtaggaa
     3121 caaaaaagtt aacaaagtca tttgggtggg aaactttaga tagcttcatg caacatgatg
     3181 ttcaggagct ttgtcgagtg ttgctcgata atgtggaaaa taagatgaaa ggcacctgtg
     3241 tagagggcac catacccaaa ttattccgcg gcaaaatggt gtcctatatc cagtgtaaag
     3301 aagtagacta tcggtctgat agaagagaag attattatga tatccagcta agtatcaaag
     3361 gaaagaaaaa tatatttgaa tcatttgtgg attatgtggc agtagaacag ctcgatgggg
     3421 acaataaata cgacgctggg gaacatggct tacaggaagc agagaaaggt gtgaaattcc
     3481 taacattgcc accagtgtta catctacaac tgatgagatt tatgtatgac cctcagacgg
     3541 accaaaatat caagatcaat gataggtttg aattcccaga gcagttacca cttgatgaat
     3601 ttttgcaaaa aacagatcct aaggaccctg caaattatat tcttcatgca gtcctggttc
     3661 atagtggaga taatcatggt ggacattatg tggtttatct aaaccccaaa ggggatggca
     3721 aatggtgtaa atttgatgac gacgtggtgt caaggtgtac taaagaggaa gcaattgagc
     3781 acaattatgg gggtcacgat gacgacctgt ctgttcgaca ctgcactaat gcttacatgt
     3841 tagtctacat cagggaatca aaactgagtg aagttttaca ggcggtcacc gaccatgata
     3901 ttcctcagca gttggtggag cgattacaag aagagaaaag gatcgaggct cagaagcgga
     3961 aggagcggca ggaagcccat ctctatatgc aagtgcagat agtcgcagag gaccagtttt
     4021 gtggccacca agggaatgac atgtacgatg aagaaaaagt gaaatacact gtgttcaaag
     4081 tattgaagaa ctcctcgctt gctgagtttg ttcagagcct ctctcagacc atgggatttc
     4141 cacaagatca aattcgattg tggcccatgc aagcaaggag taatggaaca aaacgaccag
     4201 caatgttaga taatgaagcc gacggcaata aaacaatgat tgagctcagt gataatgaaa
     4261 acccttggac aatattcctg gaaacagttg atcccgagct ggctgctagt ggagcgacct
     4321 tacccaagtt tgataaagat catgatgtaa tgttattttt gaagatgtat gatcccaaaa
     4381 cgcggagctt gaattactgt gggcatatct acacaccaat atcctgtaaa atacgtgact
     4441 tgctcccagt tatgtgtgac agagcaggat ttattcaaga tactagcctt atcctctatg
     4501 aggaagttaa accgaattta acagagagaa ttcaggacta tgacgtgtct cttgataaag
     4561 cccttgatga actaatggat ggtgacatca tagtatttca gaaggatgac cctgaaaatg
     4621 ataacagtga attacccacc gcaaaggagt atttccgaga tctctaccac cgcgttgatg
     4681 tcattttctg tgataaaaca atccctaatg atcctggatt tgtggttacg ttatcaaata
     4741 gaatgaatta ttttcaggtt gcaaagacag ttgcacagag gctcaacaca gatccaatgt
     4801 tgctgcagtt tttcaagtct caaggttata gggatggccc aggtaatcct cttagacata
     4861 attatgaagg tactttaaga gatcttctac agttcttcaa gcctagacaa cctaagaaac
     4921 tttactatca gcagcttaag atgaaaatca cagactttga gaacaggcga agttttaaat
     4981 gtatatggtt aaacagccaa tttagggaag aggaaataac actatatcca gacaagcatg
     5041 ggtgtgtccg ggacctgtta gaagaatgta aaaaggccgt ggagcttggg gagaaagcat
     5101 cagggaaact taggctgcta gaaattgtaa gctacaaaat cattggtgtt catcaagaag
     5161 atgaactatt agaatgttta tctcctgcaa cgagccggac gtttcgaata gaggaaatcc
     5221 ctttggacca ggtggacata gacaaagaga atgagatgct tgtcacagtg gcgcatttcc
     5281 acaaagaggt cttcggaacg ttcggaatcc cgtttttgct gaggatacac cagggcgagc
     5341 attttcgaga agtgatgaag cgaatccaga gcctgctgga catccaggag aaggagtttg
     5401 agaagtttaa atttgcaatt gtaatgacgg gccgacacca gtacataaat gaagacgagt
     5461 atgaagtaaa tttgaaagac tttgagccac agcccggtaa tatgtctcat cctcggcctt
     5521 ggctagggct cgaccacttc aacaaagccc caaagaggag tcgctacact taccttgaaa
     5581 aggccattaa aatccataac tgaggatccg gaattccgcc cctctccctc ccccccccct
     5641 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     5701 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     5761 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     5821 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     5881 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     5941 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6001 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6061 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6121 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6181 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc
     6241 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     6301 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     6361 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6421 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6481 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6541 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6601 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6661 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6721 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa
     6781 ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     6841 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg
     6901 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagtcgagg gctgcagcgc
     6961 tgcagaggcc gagtgcagaa ctgctccaaa gggacctcaa ggctttccga gggacactag
     7021 gctgactcca tcgagccagt gtagagataa gcttatcgat tagtccaatt tgttaaagac
     7081 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
     7141 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     7201 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     7261 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg
     7321 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     7381 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     7441 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     7501 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     7561 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     7621 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     7681 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7741 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7801 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     7861 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8041 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8101 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8161 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8221 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     8281 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     8341 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8941 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9121 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9181 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9241 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9301 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9361 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9421 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9481 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9541 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     9601 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9661 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9721 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9781 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9841 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     9901 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9961 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10021 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10081 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10141 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10201 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10261 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10321 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
    10381 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
    10441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//
