LOCUS       sequence-93115-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites
            removed and S612 restored.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF
  TITLE     Cyclin D activates the Rb tumor suppressor by mono-phosphorylation.
  JOURNAL   Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.
  PUBMED    24876129
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            May 29:e02872. doi: 10.7554/eLife.02872."
COMMENT     Sequence Label: pCMV  HA hRB delta CDK + S612
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          131..227
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             291..317
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gaaccgttca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gggaccgatc cagcctccgg actctagagg atccggtact agaggaactg aaaaaccaga
      121 aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg
      181 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag
      241 tgttacttct gctctaaaag ctgcggaatt gtacccgcgg gcccaccatg tacccatacg
      301 atgttccaga ttacgctctt atggccatgg aggcccgaat tcggtcgacc ccgcccaaag
      361 ccccccgaaa aacggccgcc accgccgccg ctgccgccgc ggaacccccg gcaccgccgc
      421 cgccgccccc tcctgaggag gacccagagc aggacagcgg cccggaggac ctgcctctcg
      481 tcaggcttga gtttgaagaa acagaagaac ctgattttac tgcattatgt cagaaattaa
      541 agataccaga tcatgtcaga gagagagctt ggttaacttg ggagaaagtt tcatctgtgg
      601 atggagtatt gggaggttat attcaaaaga aaaaggaact gtggggaatc tgtatcttta
      661 ttgcagcagt tgacctagat gagatgtcgt tcacttttac tgagctacag aaaaacatag
      721 aaatcagtgt ccataaattc
//
