LOCUS       sequence-93124-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 68bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL815N
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    47..221
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(249..265)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..265)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(293..310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(293..309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(302..324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    512..536
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgcggacgt ttttatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag
       61 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
      121 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa
      181 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagaat tcgagctcgg
      241 tacccgggga tccactagtt ctagagtcga cctgcaggca tgcaagcttg gcactggccg
      301 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      361 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      421 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
      481 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
      541 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      601 ttgg
//
