LOCUS       sequence-93136-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4NW2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC034-H11
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             659..676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(729..747)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      743..790
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg cgaagaggaa tttgaaacca
      121 tagaaagatt tatggattgt cggattggga gaaaaggagc tactggtgct actacaacca
      181 tctatgcagt tgaagcagat ggtgacccaa atgcaggctt tgaaaaaaac aaagaaccag
      241 gagagattca gtatttaatt aaatggaaag gatggtccca tatccacaac acttgggaga
      301 cagaagaaac cctcaagcag cagaatgtta gaggaatgaa aaaattggat aattataaga
      361 aaaaagatca ggaaacaaaa agatggttga aaaatgcctc tccagaagat gtggaatatt
      421 ataattgcca gcaagaactt acagatgatc tacataaaca gtatcaaata gtggaacgta
      481 taattgctca ttccaatcaa aagtcagcag ctggttatcc tgattattac tgcaaatggc
      541 agggccttcc atactcagag tgcagctggg aagatggagc tctcatttcc aaaaagtttc
      601 aagcatgcat tgatgagtat tttagcaggt gatgacgaag cttgcggccg cactcgagca
      661 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      721 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      781 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac accttgccag
      901 cgccctagcg ccccgctcct ttcgctttcc ttccccttcc ttttctcgcc cacgtt
//
