LOCUS       sequence-93137-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4NW2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC034-H11
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(604..624)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             664..686
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    701..725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(725..744)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(726..744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(828..847)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttcgt catcacctgc taaaatactc atcaatgcat gcttgaaact ttttggaaat
      121 gagagctcca tcttcccagc tgcactctga gtatggaagg ccctgccatt tgcagtaata
      181 atcaggataa ccagctgctg acttttgatt ggaatgagca attatacgtt ccactatttg
      241 atactgttta tgtagatcat ctgtaagttc ttgctggcaa ttataatatt ccacatcttc
      301 tggagaggca tttttcaacc atctttttgt ttcctgatct tttttcttat aattatccaa
      361 ttttttcatt cctctaacat tctgctgctt gagggtttct tctgtctccc aagtgttgtg
      421 gatatgggac catcctttcc atttaattaa atactgaatc tctcctggtt ctttgttttt
      481 ttcaaagcct gcatttgggt caccatctgc ttcaactgca tagatggttg tagtagcacc
      541 agtagctcct tttctcccaa tccgacaatc cataaatctt tctatggttt caaattcctc
      601 ttcgccctgg aaatacaagt tttctctgcc gctgctgtga tgatgatgat gatgcatggt
      661 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
      721 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg
      781 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      841 catcaccg
//
