LOCUS       sequence-93138-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O2W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: YTC013-A06
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgc atcatcatca
       61 tcatcacagc agcggcagag aaaacttgta tttccagggc ggcatggatg aacaaattat
      121 gtcttgggca acttccagac ctgaggactg gcacctggga ggtaaatgtg atgtctactt
      181 atggggtgct ggtaggcatg gacagctggc agaagctgga agaaatgtaa tggtacctgc
      241 agcagctccc tcattctcac aggcccaaca ggtcatttgt ggtcagaatt gtacctttgt
      301 catccaggcc aatggcacag tgttggcttg tggggaagga agttatggca gattaggaca
      361 aggaaattca gatgaccttc atgtgctgac agttatttca gccttacaag gctttgtggt
      421 gacccagctg gtgacttcct gtggttctga tgggcactct atggccctaa ctgaaagtgg
      481 tgaggtcttt agctggggag atggtgacta tggtaaactt ggccatggga acagcgacag
      541 gcagcggcgg cccaggcaga tcgaggcctt acaaggagaa gaagtggtgc agatgtcttg
      601 tggcttcaag cactcagcag tggtcacttc agatggcaaa ctgttcacct ttgggaatgg
      661 tgactatggt cgtctgggtc ttggaaatac ctctaacaaa aaacttccag agagagtgac
      721 tgcactggag ggatatcaga ttggacaggt ggcctgtgga ttaaaccaca ctttggcagt
      781 gtcagcagat ggttccatgg tgtgggcttt tggagatgga gactatggaa aactaggctt
      841 aggaaattcc actgcaaaat cttcacctca gaaaattgac gtccttt
//
