LOCUS       sequence-9314-ex        4007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC157
FEATURES             Location/Qualifiers
     source          1..4007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2132..2231)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2248..2267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2249..2267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2272..2288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2390..2409)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2401..3210
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2468..2487)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3357..3945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3846..3865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctct gcagtttgtg caacaaattg aggaaggcat tctagagaag aatttcggca
      721 tttcgcaatg atacaaaatg aagagaataa ggaaatgacg gtgaaataac cggtttgaag
      781 gtaaaaatga taaagtagac aattgaattt ttctagttca ttctccaaca ataaattcac
      841 aacaaaacaa atctgcctag ataaaaaact aaaagatata tcaagaatac atctagaatc
      901 ctttgctgta gaagaattgc tcaataagaa cacgtggagc tcctttaatt ttgatacgac
      961 ctttcaactc atcgacgtga ctggcgatcg aatgtccaag atgttcttca agataggagc
     1021 ggagatcatt ttcgcaagcc tgaaagatat ttatgttgat tcaggattta ataatgttcc
     1081 caacttacaa aaatgtctcc gtcgacgttt ctgatagtta ctagctcgac ataatcaaaa
     1141 tccaacgttt tctcattcag taaatccctt tttcgttcca aataaagagg aagaagatgg
     1201 tcatgacgac gtcgaatgta gtattgatgt gtcttggctg cttctgaaat atttcgatgc
     1261 ggtatgaatt taatctgtat atacctgtcg gtggtgtcca tccgcttggt gtaggatact
     1321 tttcgtgctc aggaacattc ggcaccactt ctattggcat caatctttcc acatacgacc
     1381 aatcgatagg agcttcttcg accgttgcaa gacttctggc tgtaattgct agtagaaaaa
     1441 ttatatgata ataaataact tacacttttc agggagagca tgtttccatg gattttccca
     1501 aagtttctca gctttttcga ctgaagttgt tgaaaaagac aacctggaaa ctgctcgagg
     1561 ggagaaaatg cggagagaag aacccagcat cttaataaaa ttatataatt ttgaataaaa
     1621 tcaaattaaa aataagatta attgaaacac aacaatagaa tgcagaaaat tactcataag
     1681 aaataattta ttttgaaaga agttgtgtcg atttacgata aaaactacac gcaaacgagg
     1741 ctccgcgttt acaccgtttg gcaatggagc gcgcttgcta ttagatttta ggccagctca
     1801 acgaaagttt aatttatttt tcttcttttt ttccattttt ttgagtaaaa tatcgaattt
     1861 tttgtattaa ttctgtaaat tttataattt gcaactattt tggcgtattc tgcttgtttt
     1921 tatcagcgaa tttttatgca tttcagtcaa aagtttgagc aggaaaaatg acatttagga
     1981 ctttgtaaaa ttgtattctt aaatttgctt gaaaacctac gtgtacaatt gattttattg
     2041 aatttttctt acgtttcccg ttgtatctta ttgtatttaa ctttcatcat aaaataattt
     2101 atttccaggt aaaatatccg acgctctcgt gacccagctt tcttgtacaa agttggcatt
     2161 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2221 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2281 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2341 ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2401 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2461 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2521 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2581 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2641 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2701 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2761 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2821 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2881 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2941 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     3001 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     3061 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3121 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3181 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3241 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3301 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
