LOCUS       sequence-93141-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O62.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC01B-H12
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             275..292
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(345..363)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      359..406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      443..825
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(530..549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     740..761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taagaaggag atataccatg catcatcatc atcatcacag cagcggcaga gaaaacttgt
       61 atttccaggg cgtggaaaac atgtatgtaa acaaagtgtg ggttcaatgt gagaatgaaa
      121 attgtttgaa atggagattg ttatcaagtg aggattcagc caaggttgat catgatgaac
      181 catggtactg cttcatgaac actgattcaa gatataataa ctgctcaatt tctgaagaag
      241 acttctgatg acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      301 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      361 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      421 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      481 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      721 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      781 tcggtctatt ctttttgatt tataaaggga ttttgccgat tttcggccta ttggttaaaa
      841 aatgagcttg atttaaacaa aaatttaac
//
