LOCUS       sequence-93143-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O45 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: APC041-G05
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgc atcatcatca
       61 tcatcacagc agcggcagag aaaacttgta tttccagggc actcagagca agcctacacc
      121 tgtgaagcca aactatgctc taaagttcac ccttgctggc cacaccaaag cagtgtcctc
      181 cgtgaaattc agcccgaatg gagagtggct ggcaagttca tctgctgata aacttattaa
      241 aatttggggc gcgtatgatg ggaaatttga gaaaaccata tctggtcaca agctgggaat
      301 atccgatgta gcctggtcgt cagattctaa ccttcttgtt tctgcctcag atgacaaaac
      361 cttgaagata tgggacgtga gctcgggcaa gtgtctgaaa accctgaagg gacacagtaa
      421 ttatgtcttt tgctgcaact tcaatcccca gtccaacctt attgtctcag gatcctttga
      481 cgaaagcgtg aggatatggg atgtgaaaac agggaagtgc ctcaagactt tgccagctca
      541 ctcggatcca gtctcggccg ttcattttaa tcgtgatgga tccttgatag tttcaagtag
      601 ctatgatggt ctctgtcgca tctgggacac cgcctcgggc cagtgcctga agacgctcat
      661 cgatgacgac aacccccccg tgtcttttgt gaagttctcc ccgaacggca aatacatcct
      721 ggccgccacg ctggacaaca ctctgaagct ctgggactac agcaagggga agtgcctgaa
      781 gacgtacact ggccacaaga atgagaaata ctgcatattt gccaatttct ctgttactgg
      841 tgggaagtgg attgtgtctg gctcagagga taaccttgtt tacatctgga accttcagac
      901 gaaagagatt gtacagaaac tacaaggcca cacagatgtc gtgatctcaa cagcttgtca
      961 cccaacagaa aacatcatcg cct
//
