LOCUS       sequence-93144-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 548, Emission:
            562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mOrange-pBAD
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..31
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(35..43,44..58)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     487..506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga
       61 tccgagctcg agcatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt
      121 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      181 cgagggcgag ggccgcccct acgagggctt tcagaccgct aagctgaagg tgaccaaggg
      241 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcacctacg gctccaaggc
      301 ctacgtgaag caccccgccg acatccccga ctacttcaag ctgtccttcc ccgagggctt
      361 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      421 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      481 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      541 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
      601 ccactacacc tccgaggt
//
