LOCUS       sequence-93146-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O64 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC077-F02
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(552..570)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      566..613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg ccggcggcgc cggacgcgat
      121 gccgcaagtg cgaggcttgt ttacgtactg aatgtggtga atgtcacttc tgtaaagata
      181 tgaaaaaatt cggtggtcca ggtcgtatga aacaatcttg tattatgcgt caatgtattg
      241 ctccagtttt accacacact gctgtttgtt tagtttgtgg tgaagctggt aaagaagata
      301 ctgttgaaga agaagaaggt aaattcaact taatgttaat ggaatgttct atttgtaacg
      361 aaattattca cccaggttgt ttaaaaatta aagaatctga aggtgttgtt aacgatgaat
      421 taccaaactg ttgggagtgt ccgaagtgta actgatgacg aagcttgcgg ccgcactcga
      481 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      601 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcggggggct ccctttaggg ttccgattta gtgctttacg
      841 gcacctcgac ccccaaaaaa cttgatttag ggtgatggtt cacgtagtgg gccatcgccc
      901 ctgatagacg gtttttcgcc ctttga
//
