LOCUS       sequence-93147-e         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O64 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JMC077-F02
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(407..427)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(440..457)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             467..489
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    504..528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttcgt catcagttac
       61 acttcggaca ctcccaacag tttggtaatt catcgttaac aacaccttca gattctttaa
      121 tttttaaaca acctgggtga ataatttcgt tacaaataga acattccatt aacattaagt
      181 tgaatttacc ttcttcttct tcaacagtat cttctttacc agcttcacca caaactaaac
      241 aaacagcagt gtgtggtaaa actggagcaa tacattgacg cataatacaa gattgtttca
      301 tacgacctgg accaccgaat tttttcatat ctttacagaa gtgacattca ccacattcag
      361 tacgtaaaca agcctcgcac ttgcggcatc gcgtccggcg ccgccggccc tggaaataca
      421 agttttctct gccgctgctg tgatgatgat gatgatgcat ggtatatctc cttcttaaag
      481 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      541 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      601 tcaccggcgc ca
//
