LOCUS       sequence-93148-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, 
            Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, 
            Chudakov DM
  TITLE     Bright far-red fluorescent protein for whole-body imaging.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):741-6.
  PUBMED    17721542
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):741-6."
COMMENT     Sequence Label: dKatushka-pBAD
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             43..65
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             106..138
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             151..171
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctactgttt ctccataccc gtttttttgg gctagaaata attttgttta actttaagaa
       61 ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc tagcatgact
      121 ggtggacagc aaatgggtcg ggatctgtac gagaacctgt acttccaggg ctcgagcatg
      181 gtgggtgagg atagcgtgct gatcaccgag aacatgcaca tgaaactgta catggagggc
      241 accgtgaacg accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      301 acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      361 ctggctacca gcttcatgta cggcagcaaa acctttatca accacaccca gggcatcccc
      421 gacttcttta agcagtcctt ccctgagggc ttcacatggg agaggatcac cacatacgaa
      481 gacgggggcg tgctgaccgc tacccaggac accagcctcc agaacggctg cctcatctac
      541 aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
      601 ctcggctggg aggccagcac cgagatgctg taccccgctg acagcggcct gagaggccat
      661 agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctccct caagaccaca
      721 tacagatcca aga
//
