LOCUS       sequence-93150-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4PWY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PBC002-A04
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taagaaggag atataccatg catcatcatc atcatcacag cagcggcaga gaaaacttgt
       61 atttccaggg ctctgtaaga agatttgaat catttaatct gttttcagta acagaaggca
      121 aagaaaggga aactgaagag gaggttggtg catgggtcca atatacaagc atcttctgtc
      181 ctgaatacag tatctcctta aggcataata gtggatcctt gaatgttgaa gatgtcctta
      241 ccagctttga caatacagga aatgtttgca tctggccatc tgaagaggtt ttggcttact
      301 actgcctcaa gcacaataat atattcaggg cccttgctgt gtgtgagcta gggggtggca
      361 tgacatgctt ggctgggctc atggttgcta tttctgcaga tgtcaaagaa gttctgttaa
      421 ctgatgggaa tgaaaaggcc atcagaaatg tgcaagacat catcacaagg aatcagaagg
      481 ctggtgtgtt taagacccag aaaatatcaa gctgcttttt acgatgggat aatgagacag
      541 atgtctctca actggaagga cattttgaca ttgttatgtg tgctgactgc ctgtttctgg
      601 accagtacag agccagcctt gttgatgcaa taaagagatt actccagccc agggggaaag
      661 cgatggtatt tgccccacgc cgagggaata ctttaaacca gttttgcaat ctagctgaaa
      721 aagctggttt ctgtatccaa agacatgaaa attatgatga acacatttca aacttccact
      781 ccaagttgaa aaaggaaaac ccggacatat atgaagaaaa ccttcattac ccgcttctgc
      841 ttattttgac caaacatgga tgatgacgaa gcttgcggcc gcactcgagc acacccacca
      901 ccaccactga gatccggctg ctacaaagcc cgaaaggaag ctga
//
