LOCUS       sequence-93151-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4PWY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PBC002-A04
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(867..887)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttc gtcatcatcc atgtttggtc aaaataagca gaagcgggta atgaaggttt
      121 tcttcatata tgtccgggtt ttcctttttc aacttggagt ggaagtttga aatgtgttca
      181 tcataatttt catgtctttg gatacagaaa ccagcttttt cagctagatt gcaaaactgg
      241 tttaaagtat tccctcggcg tggggcaaat accatcgctt tccccctggg ctggagtaat
      301 ctctttattg catcaacaag gctggctctg tactggtcca gaaacaggca gtcagcacac
      361 ataacaatgt caaaatgtcc ttccagttga gagacatctg tctcattatc ccatcgtaaa
      421 aagcagcttg atattttctg ggtcttaaac acaccagcct tctgattcct tgtgatgatg
      481 tcttgcacat ttctgatggc cttttcattc ccatcagtta acagaacttc tttgacatct
      541 gcagaaatag caaccatgag cccagccaag catgtcatgc caccccctag ctcacacaca
      601 gcaagggccc tgaatatatt attgtgcttg aggcagtagt aagccaaaac ctcttcagat
      661 ggccagatgc aaacatttcc tgtattgtca aagctggtaa ggacatcttc aacattcaag
      721 gatccactat tatgccttaa ggagatactg tattcaggac agaagatgct tgtatattgg
      781 acccatgcac caacctcctc ttcagtttcc ctttctttgc cttctgttac tgaaaacaga
      841 ttaaatgatt caaatcttct tacagagccc tggaaataca agttttctct gccgctgc
//
