LOCUS       sequence-93156-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4PYZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SDC229-B03
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..74
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
       61 ttccgcgtgg tagtcctcag cagttggtgg agcgattaca agaagagaaa aggatcgagg
      121 ctcagaagcg gaaggagcgg caggaagccc atctctatat gcaagtgcag atagtcgcag
      181 aggaccagtt ttgtggccac caagggaatg acatgtacga tgaagaaaaa gtgaaataca
      241 ctgtgttcaa agtattgaag aactcctcgc ttgctgagtt tgttcagagc ctctctcaga
      301 ccatgggatt tccacaagat caaattcgat tgtggcccat gcaagcaagg agtaatggaa
      361 caaaacgacc agcaatgtta gataatgaag ccgacggcaa taaaacaatg attgagctca
      421 gtgataatga aaacccttgg acaatattcc tggaaacagt tgatcccgag ctggctgcta
      481 gtggagcgac cttacccaag tttgataaag atcatgatgt aatgttattt ttgaagatgt
      541 atgatcccaa aacgcggagc ttgaattact gtgggcatat ctacacacca atatcctgta
      601 aaatacgtga cttgctccca gttatgtgtg acagagcagg atttattcaa gatactagcc
      661 ttatcctcta tgaggaagtt aaaccgaatt taacagagag aattcaggac tatgacgtgt
      721 ctcttgataa agcccttgat gaactaatgg atggtgacat catagtattt cagaaggatg
      781 accctgaaaa tgataacagt gaattaccca ccgcaaagga gtatttccga gatctctacc
      841 accgctgatg acgaaagctt gcggccgcac tcgagcacca ccacc
//
