LOCUS       sequence-93157-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: mKate1.31-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagcg agctgattaa
      121 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
      181 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt
      241 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
      301 caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga
      361 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      421 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
      481 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac
      541 cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg
      601 cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa
      661 cctcaagatg cccggcgtct actatgtgga cagaagactg gaa
//
