LOCUS       sequence-93161-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Dronpa3-N1
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..782
                     /codon_start=1
                     /product="photoswitchable Dronpa-Green3 fluorescent 
                     protein"
                     /label=Dronpa-Green3
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDINAALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agtgtgatta
      121 aaccagacat gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga
      181 ttgaaggagt tggccttggg aagcctttcg agggaaaaca gagtatggac cttaaagtca
      241 aagaaggcgg acctctgcct ttcgcctatg acatcttgac aactgtgttc tgttacggca
      301 acagggtatt cgccaaatac ccagaaaata tagtagacta tttcaagcag tcgtttcctg
      361 agggctactc ttgggaacga agcatgaatt acgaagacgg gggcatttgt aacgcgacaa
      421 acgacataac cctggatggt gactgttata tctatgaaat tcgatttgat ggtgtgaact
      481 ttcctgccaa tggtccagtt atgcagaaga ggactgtgaa atgggagcca tccactgaga
      541 aattgtatgt gcgtgatgga gtgctgaagg gtgatattaa cgcggctctg tcgcttgaag
      601 gaggtggcca ttaccgatgt gacttcaaaa ctacttataa agctaagaag gttgtccagt
      661 tgccagacta tcactttgtg gaccaccaca ttgagattaa aagccacgac aaagattaca
      721 gtaatgttaa tctgcatgag cacgccgaag cgcattctga gctgccgagg caggccaagt
      781 aatgataggc ggccgcgact ctagatcata atcagccata ccacatttgt agaggttt
//
