LOCUS       sequence-93163-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 453, Emission: 486.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AmCyan1-N1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    4..56
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..759
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
ORIGIN
        1 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
       61 gtcgccacca tggccctgtc caacaagttc atcggcgacg acatgaagat gacctaccac
      121 atggacggct gcgtgaacgg ccactacttc accgtgaagg gcgagggcag cggcaagccc
      181 tacgagggca cccagacctc caccttcaag gtgaccatgg ccaacggcgg ccccctggcc
      241 ttctccttcg acatcctgtc caccgtgttc atgtacggca accgctgctt caccgcctac
      301 cccaccagca tgcccgacta cttcaagcag gccttccccg acggcatgtc ctacgagaga
      361 accttcacct acgaggacgg cggcgtggcc accgccagct gggagatcag cctgaagggc
      421 aactgcttcg agcacaagtc caccttccac ggcgtgaact tccccgccga cggccccgtg
      481 atggccaaga agaccaccgg ctgggacccc tccttcgaga agatgaccgt gtgcgacggc
      541 atcttgaagg gcgacgtgac cgccttcctg atgctgcagg gcggcggcaa ctacagatgc
      601 cagttccaca cctcctacaa gaccaagaag cccgtgacca tgccccccaa ccacgtggtg
      661 gagcaccgca tcgccagaac cgacctggac aagggcggca acagcgtgca gctgaccgag
      721 cacgccgtgg cccacatcac ctccgtggtg cccttctaag cggccgcgac tctagatcat
      781 aatcagccat accacatt
//
