LOCUS       sequence-93167-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 4.5 kb promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh4.5k-Luc
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    149..283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    403..537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    657..738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 agcttgcaga tctgcgactc tagaggatct gcgactctag aggatcataa tcagccatac
       61 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      121 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      241 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgcgact ctagaggatc
      301 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      361 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc
      541 gactctagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      601 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      661 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      721 aagcattttt ttcactgcat tctagttgtg gtttgtccaa a
//
