LOCUS       sequence-93176-e        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of APX W41F inducible by IPTG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Dwyer DJ, Belenky PA, Yang JH, MacDonald IC, Martell JD, Takahashi 
            N, Chan CT, Lobritz MA, Braff D, Schwarz EG, Ye JD, Pati M, 
            Vercruysse M, Ralifo PS, Allison KR, Khalil AS, Ting AY, Walker GC, 
            Collins JJ
  TITLE     Antibiotics induce redox-related physiological alterations as part 
            of their lethality.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 
            10.1073/pnas.1401876111. Epub 2014 May 6.
  PUBMED    24803433
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401876111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-20- 20"; volume: "111"; issue: "20"; pages: "E2100-9"
COMMENT     Sequence Label: pTRC-APX(W41F) dimeric
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(571..576,577..593,594..600)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    608..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     613..635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             651..668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
       61 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
      121 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
      181 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
      241 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      301 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag
      361 ttagcgcgaa ttgatctggt ttgacagctt atcatcgact gcacggtgca ccaatgcttc
      421 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata
      481 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
      541 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc tcgtataatg
      601 tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatggca caccaccacc
      661 accaccacaa ggatccagga aagtcatacc caaccgtgag cccagattac cagaaggcta
      721 ttgagaaggc aaaaaggaaa ctgaggggat tcatcgcaga aaagaaatgc gccccactga
      781 tcctgcgact ggctttccac agcgcaggaa catttgactc caagaccaaa acaggcggac
      841 cctttgggac tatcaagcac caggctgaac tggcacatgg ggccaacaat ggcctggaca
      901 ttgctgtgag actgctggag ccaatcaaag aacagttccc cattgtctcc tacgcagact
      961 tctaccagct ggccggcgtg gtcgctgtgg agattaccgg cggccccgag gtccctttcc
     1021 acccaggaag ggaggacaaa cctgaacccc ctccagaggg gagactgcca gatgctacca
     1081 agggcagcga ccatctgcgg gacgtgttcg gaaaa
//
