LOCUS       sequence-93180-e        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of APX W41F inducible by IPTG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Dwyer DJ, Belenky PA, Yang JH, MacDonald IC, Martell JD, Takahashi 
            N, Chan CT, Lobritz MA, Braff D, Schwarz EG, Ye JD, Pati M, 
            Vercruysse M, Ralifo PS, Allison KR, Khalil AS, Ting AY, Walker GC, 
            Collins JJ
  TITLE     Antibiotics induce redox-related physiological alterations as part 
            of their lethality.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 
            10.1073/pnas.1401876111. Epub 2014 May 6.
  PUBMED    24803433
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401876111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-20- 20"; volume: "111"; issue: "20"; pages: "E2100-9"
COMMENT     Sequence Label: pTRC-APX(W41F) dimeric
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..134
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(966..983)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(999..1021)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1010..1026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1034..1040,1041..1057,1058..1063))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
       61 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
      121 taatctgtat caggctgaaa atcttctctc atccgccaaa acagccaagc ttgcctgcag
      181 gtcgactcta gaggatctta agaattccct gcctcggcga atcccagttc agacagtttc
      241 aggtgtgcct cagcatagtc ggcgaagaac acatcctcgt cggctgcata cttttcgacc
      301 agaggcctga acacgctatc tgtcagcagg gctttgtcag agggcagctg cagcagtcca
      361 tccttctcgc cggtcagcag ttcagtaaag taggaattgt cgaagatcag agggttgctt
      421 gtccagggtc cctcgaagcc tgagcgttcc ttatgagcgg cgccaatggt gtgccctcca
      481 ctcagggcca cgatatcctg gtcagacagc cccattgctt ttccgaacac gtcccgcaga
      541 tggtcgctgc ccttggtagc atctggcagt ctcccctctg gagggggttc aggtttgtcc
      601 tcccttcctg ggtggaaagg gacctcgggg ccgccggtaa tctccacagc gaccacgccg
      661 gccagctggt agaagtctgc gtaggagaca atggggaact gttctttgat tggctccagc
      721 agtctcacag caatgtccag gccattgttg gccccatgtg ccagttcagc ctggtgcttg
      781 atagtcccaa agggtccgcc tgttttggtc ttggagtcaa atgttcctgc gctgtggaaa
      841 gccagtcgca ggatcagtgg ggcgcatttc ttttctgcga tgaatcccct cagtttcctt
      901 tttgccttct caatagcctt ctggtaatct gggctcacgg ttgggtatga ctttcctgga
      961 tccttgtggt ggtggtggtg gtgtgccatg gtctgtttcc tgtgtgaaat tgttatccgc
     1021 tcacaattcc acacattata cgagccggat gattaattgt caacagctca tttcagaata
     1081 tttgccagaa ccgttatgat gtcggcgcaa a
//
