LOCUS       sequence-93186-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of APX W41F under control of TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Dwyer DJ, Belenky PA, Yang JH, MacDonald IC, Martell JD, Takahashi 
            N, Chan CT, Lobritz MA, Braff D, Schwarz EG, Ye JD, Pati M, 
            Vercruysse M, Ralifo PS, Allison KR, Khalil AS, Ting AY, Walker GC, 
            Collins JJ
  TITLE     Antibiotics induce redox-related physiological alterations as part 
            of their lethality.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 
            10.1073/pnas.1401876111. Epub 2014 May 6.
  PUBMED    24803433
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401876111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-20- 20"; volume: "111"; issue: "20"; pages: "E2100-9"
COMMENT     Sequence Label: pZE21-APX(W41F) dimeric
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     221..239
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(260..278,279..284,285..301,302..307,308..333)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             343..354
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
       61 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
      121 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga
      181 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
      241 ctttcgtctt cacctcgagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      301 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
      361 atgggaaagt catacccaac cgtgagccca gattaccaga aggctattga gaaggcaaaa
      421 aggaaactga ggggattcat cgcagaaaag aaatgcgccc cactgatcct gcgactggct
      481 ttccacagcg caggaacatt tgactccaag accaaaacag gcggaccctt tgggactatc
      541 aagcaccagg ctgaactggc acatggggcc aacaatggcc tggacattgc tgtgagactg
      601 ctggagccaa tcaaagaaca gttccccatt gtctcctacg cagacttcta ccagctggcc
      661 ggcgtggtcg ctgtggagat taccggcggc cccgaggtcc ctttccaccc aggaagggag
      721 gacaaacctg aaccccctcc agaggggaga ctgccagatg ctaccaaggg cagcgaccat
      781 ctgcgggacg tgttcggaaa agcaatgggg ctgtctgacc aggatatcgt ggccctgagt
      841 ggagggcaca ccattggcgc cgctcataag gaacgctcag gcttcgaggg accctggaca
      901 agcaaccctc tgatcttcga caattcctac tttactgaac tgctgaccgg cgagaaggat
      961 ggactgctgc agctgccctc tgacaaagcc ctgctgacag atagcgtgtt caggcctctg
     1021 gtcgaaaagt atgcagccga cgaggatgtg ttcttcgccg actatg
//
