LOCUS       sequence-93187-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of APX W41F under control of TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Dwyer DJ, Belenky PA, Yang JH, MacDonald IC, Martell JD, Takahashi 
            N, Chan CT, Lobritz MA, Braff D, Schwarz EG, Ye JD, Pati M, 
            Vercruysse M, Ralifo PS, Allison KR, Khalil AS, Ting AY, Walker GC, 
            Collins JJ
  TITLE     Antibiotics induce redox-related physiological alterations as part 
            of their lethality.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 
            10.1073/pnas.1401876111. Epub 2014 May 6.
  PUBMED    24803433
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401876111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-20- 20"; volume: "111"; issue: "20"; pages: "E2100-9"
COMMENT     Sequence Label: pZE21-APX(W41F) dimeric
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..96)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             915..926
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(936..961,962..967,968..984,985..990,
                     991..1009))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    966..984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    991..1009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
       61 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctagcacg cgtaccatgg
      121 gatcccccgg gctgcaggaa ttcgatatca agcttctatg cctcggcgaa tcccagttca
      181 gacagtttca ggtgtgcctc agcatagtcg gcgaagaaca catcctcgtc ggctgcatac
      241 ttttcgacca gaggcctgaa cacgctatct gtcagcaggg ctttgtcaga gggcagctgc
      301 agcagtccat ccttctcgcc ggtcagcagt tcagtaaagt aggaattgtc gaagatcaga
      361 gggttgcttg tccagggtcc ctcgaagcct gagcgttcct tatgagcggc gccaatggtg
      421 tgccctccac tcagggccac gatatcctgg tcagacagcc ccattgcttt tccgaacacg
      481 tcccgcagat ggtcgctgcc cttggtagca tctggcagtc tcccctctgg agggggttca
      541 ggtttgtcct cccttcctgg gtggaaaggg acctcggggc cgccggtaat ctccacagcg
      601 accacgccgg ccagctggta gaagtctgcg taggagacaa tggggaactg ttctttgatt
      661 ggctccagca gtctcacagc aatgtccagg ccattgttgg ccccatgtgc cagttcagcc
      721 tggtgcttga tagtcccaaa gggtccgcct gttttggtct tggagtcaaa tgttcctgcg
      781 ctgtggaaag ccagtcgcag gatcagtggg gcgcatttct tttctgcgat gaatcccctc
      841 agtttccttt ttgccttctc aatagccttc tggtaatctg ggctcacggt tgggtatgac
      901 tttcccatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg ctgatgtgct
      961 cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac tcgaggtgaa
     1021 gacgaaaggg cctcgtga
//
